100× Objective Lens - Imaging at the Diffraction Limit (~100 nm Features)

The Mounted LEDs 100× oil-immersion objective delivers true diffraction-limited optical performance, achieving imaging capability down to approximately 100 nm feature scales. The lens is compatible with all commercial optical microscopes. A thread adapter might be needed.

With a 0.23 mm working distance, the objective combines high-NA performance with convenient clearance for a wide range of sample types, including reflective and nanostructured surfaces.

This has been experimentally verified by visualising the periodic groove structure of a Blu-ray disc in reflection mode(100 nm groove spacing, 200 nm track pitch) and by resolving 90 nm calcium carbonate nanoparticles (PlasmoChem GmbH) in transmission mode.

The objective operates with standard immersion oils (no proprietary medium required). Each lens is supplied with a Blu-ray reference sample and a set of 90 nm calcium carbonate nanoparticles to allow customers to reproduce and verify the lens’s performance.

Technical details:

1.Magnification: 100X

2. Numerical aperture: 1.25

3. Working distance: 0.23 mm

4. Resolution: Operates at the practical diffraction limit of visible light, enabling clear visualisation of ~100 nm structures.

5. Thread: M25 (an adapter might be required for your microscope)

6. Parfocal distance: 60 mm

7.Wavelength range: VIS

8. Immersion medium: immersion oil

Left — optical image acquired using the Mounted LEDs 100×/1.25 NA diffraction-limited objective, showing clear resolution of the 100 nm groove spacing and 200 nm track pitch (reflection mode).
Right — corresponding SEM micrograph of the same Blu-ray surface for reference.

90 nm Calcium Carbonate nanoparticles images with our novel objective lens (transmission mode)

What is included ?

100×/1.25 NA Oil-Immersion Objective Lens – precision-engineered, diffraction-limited optics.
Blu-ray Calibration Sample – features ~100 nm grooves and 200 nm track pitch for resolution testing.
90 nm Calcium Carbonate Nanoparticles (PlasmoChem GmbH) – for verifying sub-100 nm imaging capability.

Zinc oxide (ZnO) nanowires, 90 nm in diameter and 1 µm in length, imaged in transmission mode (white light illumination). The nanowires were supplied by Sigma-Aldrich (Product Code: 773999-500MG)