100× Objective Lens - Imaging at the Diffraction Limit (~100 nm Features)
Achieve true diffraction-limited optical imaging down to 100 nm.
The Mounted LEDs 100× objective lens delivers exceptional diffraction-limited performance, enabling the visualisation of structural features as small as ~100 nm on standard optical microscopes. The lens fits all major microscope brands, with optional thread adapters available when required.
With a working distance of 0.23 mm, this objective combines high numerical aperture performance with ample clearance, allowing easy imaging of reflective, nanostructured, and delicate samples.
Its performance has been experimentally verified through several benchmark tests:
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Imaging the periodic groove structure of a Blu-ray disc in white light reflection mode (100 nm grooves, 200 nm track pitch).
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Resolving 90 nm calcium carbonate nanoparticles (PlasmoChem GmbH) in transmission mode (white light illumination).
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Imaging zinc oxide nanorods (90 nm diameter × 1 µm length, Sigma-Aldrich Product Code 773999-500MG) under transmission white light illumination.
Each objective is individually tested and supplied with a Blu-ray reference sample enabling users to reproduce and validate the lens’s performance in their own lab. Additional test samples are available upon request.

On the left, an optical image captured using the Mounted LEDs 100× oil-immersion objective, clearly resolving the 100 nm grooves and 200 nm track pitch of a Blu-ray disc in reflection mode.On the right, the corresponding scanning electron microscope (SEM) image is shown for reference.

Zinc oxide (ZnO) nanowires, 90 nm in diameter and 1 µm in length, imaged in transmission mode (white light illumination).

In optical microscopy, single colour illumination has several advantages outside the fluorescence applications
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To achieve higher resolution in optical microscopy, using blue light illumination in beneficial because it has a shorter wavelength.
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While the image may appear yellowish under certain conditions, the key to a more natural appearance is lowering the wavelength of the light source.
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Achromat and Plan Achromat microscope objective lenses are best corrected spherically for green light.
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Phase contrast objective lenses are also designed to give the best phase images using green illumination.
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Certain colours increase the intensity and the saturation of red objects.
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Certain colours of illumination can enhance the intensity and saturation of red-stained objects, which is particularly important in life science imaging where tissues are stained with specific dyes. Using coloured illumination, we ensure the stained colours appear vividly, combating the "washed-out" effect.
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Techniques such as differential interference contrast (DIC) often rely on single colour illumination to enhance the interference patterns and accurately measure optical path differences.
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Red light has the longest wavelength, however, it efficiently reduces the background noise.
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Monochromatic light avoids chromatic aberrations in lens systems, enhancing resolution and image clarity.
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Single colour illumination is essential for generating clear interference patterns in digital holography. It aids in reconstructing 3D images of specimens with high spatial resolution.
Monochromatic light enhances contrast and resolution in polarised light microscopy, used to study anisotropic materials such as crystals, fibers or stressed polymers
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