
In optical microscopy, single colour illumination has several advantages outside the fluorescence applications
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To achieve higher resolution in optical microscopy, using blue light illumination in beneficial because it has a shorter wavelength.
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While the image may appear yellowish under certain conditions, the key to a more natural appearance is lowering the wavelength of the light source.
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Achromat and Plan Achromat microscope objective lenses are best corrected spherically for green light.
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Phase contrast objective lenses are also designed to give the best phase images using green illumination.
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Certain colours increase the intensity and the saturation of red objects.
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Certain colours of illumination can enhance the intensity and saturation of red-stained objects, which is particularly important in life science imaging where tissues are stained with specific dyes. Using coloured illumination, we ensure the stained colours appear vividly, combating the "washed-out" effect.
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Techniques such as differential interference contrast (DIC) often rely on single colour illumination to enhance the interference patterns and accurately measure optical path differences.
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Red light has the longest wavelength, however, it efficiently reduces the background noise.
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Monochromatic light avoids chromatic aberrations in lens systems, enhancing resolution and image clarity.
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Single colour illumination is essential for generating clear interference patterns in digital holography. It aids in reconstructing 3D images of specimens with high spatial resolution.
Monochromatic light enhances contrast and resolution in polarised light microscopy, used to study anisotropic materials such as crystals, fibers or stressed polymers
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